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Part:BBa_K1844005

Designed by: David Lips   Group: iGEM15_Amsterdam   (2015-09-20)


acs KO construct for Synechocystis

This construct includes a sequence that will target and knock out the native acetyl-CoA Synthase (acs) gene in Synechocystis via homologous recombination

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1002
    Illegal SpeI site found at 784
    Illegal SpeI site found at 1791
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 784
    Illegal SpeI site found at 1791
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1002
    Illegal SpeI site found at 784
    Illegal SpeI site found at 1791
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1002
    Illegal SpeI site found at 784
    Illegal SpeI site found at 1791
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None